Phytochemistry, Antioxidant Activity and Cytotoxicity Evaluation of Black-White Fungus Auricularia sp. against Breast MCF-7 Cancer Cells

Introduction: Cancer is a non-communicable disease with over 18.1 million new cases and 9.6 deaths annually according to WHO. Breast cancer the number two highest count type of trailing behind lung cancer. Treating expensive have various side effects. Active components found in plants or fungi that antioxidant cytotoxic activity towards cells, could be an alternative for anticancer. One potentially developed as anticancer, are genus Auricularia sp. also known black-white fungus. This study aims determine phytochemicals components, effect MCF-7 breast cells. Methods: Dried fungus grinded into fine powder. Then, multilevel maceration done n-hexane, ethyl acetate, ethanol solvents. The extracts undergo phytochemical screening thin layer chromatography (TLC), followed by measuring evaluating Results: contained secondary metabolites flavonoids, alkaloids, triterpenoids total 17 other components. Ethyl acetate extract showed weak DPPH free radical IC50 215.51 μg/mL very active on cells 0.21 μg/mL. On hand, n-hexane categorized 29.28 50.39 μg/mL, respectively. Conclusion: Black-white had anticancer should considered treatment therapy. Key words: Phytochemistry, Antioxidant, Cytotoxicity, sp.,